Large-scale purification and acute toxicity of hygromycin B phosphotransferase.
- Author:
Qin ZHUO
1
;
Jian-Hua PIAO
;
Yuan TIAN
;
Jie XU
;
Xiao-Guang YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bacterial Proteins; isolation & purification; toxicity; Escherichia coli; genetics; metabolism; Female; Male; Mice; Mice, Inbred Strains; Phosphotransferases (Alcohol Group Acceptor); isolation & purification; toxicity; Recombinant Proteins; isolation & purification; toxicity; Toxicity Tests, Acute
- From: Biomedical and Environmental Sciences 2009;22(1):22-27
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo provide the acute toxicity data of hygromycin B phosphotransferase (HPT) using recombinant protein purified from E. coli.
METHODSRecombinant HPT protein was expressed and purified from E. coli. To exclude the potential adverse effect of bacteria protein in recombinant HPT protein, bacterial control plasmid was constructed, and bacteria control protein was extracted and prepared as recombinant HPT protein. One hundred mice, randomly assigned to 5 groups, were administrated 10 g/kg, 5 g/kg, or 1 g/kg body weight of HPT or 5 g/kg body weight of bacterial control protein or phosphate-buffered saline (PBS) respectively by oral gavage.
RESULTSAll animals survived with no significant change in body weight gain throughout the study. Macroscopic necropsy examination on day 15 revealed no gross pathological lesions in any of the animals. The maximum tolerated dose (MTD) of HPT was 10 g/kg body weight in mice and could be regarded as nontoxic.
CONCLUSIONHPT protein does not have any safety problems to human health.
