Curcumin, a potential inhibitor of up-regulation of TNF-alpha and IL-6 induced by palmitate in 3T3-L1 adipocytes through NF-kappaB and JNK pathway.
- Author:
Shao-Ling WANG
1
;
Ying LI
;
Ying WEN
;
Yan-Feng CHEN
;
Li-Xin NA
;
Song-Tao LI
;
Chang-Hao SUN
Author Information
- Publication Type:Journal Article
- MeSH: 3T3-L1 Cells; Animals; Anthracenes; pharmacology; Anti-Inflammatory Agents, Non-Steroidal; pharmacology; Curcumin; pharmacology; Glucose; metabolism; Insulin; pharmacology; Insulin Resistance; Interleukin-6; genetics; metabolism; JNK Mitogen-Activated Protein Kinases; metabolism; MAP Kinase Signaling System; Mice; NF-kappa B; metabolism; Palmitates; pharmacology; Protein Kinase Inhibitors; pharmacology; Tumor Necrosis Factor-alpha; genetics; metabolism; Up-Regulation
- From: Biomedical and Environmental Sciences 2009;22(1):32-39
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the attenuating effect of curcumin, an anti-inflammatory compound derived from dietary spice turmeric (Curcuma longa) on the pro-inflammatory insulin-resistant state in 3T3-L1 adipocytes.
METHODSGlucose uptake rate was determined with the [3H] 2-deoxyglucose uptake method. Expressions of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) were measured by quantitative RT-PCR analysis and ELISA. Nuclear transcription factor kappaB p65 (NF-kappa p65) and mitogen-activated protein kinase (MAPKs) were detected by Western blot assay.
RESULTSThe basal glucose uptake was not altered, and curcumin increased the insulin-stimulated glucose uptake in 3T3-L1 cells. Curcumin suppressed the transcription and secretion of TNF-alpha and IL-6 induced by palmitate in a concentration-dependent manner. Palmitate induced nuclear translocation of NF-kappaB. The activities of Jun NH2-terminal kinase (JNK), extracellular signal-regulated kinase1/2 (ERK1/2) and p38MAPK decreased in the presence of curcumin. Moreover, pretreatment with SP600125 (inhibitor of JNK) instead of PD98059 or SB203580 (inhibitor of ERK1/2 or p38MAPK, respectively) decreased the up-regulation of TNF-alpha induced by palmitate.
CONCLUSIONCurcumin reverses palmitate-induced insulin resistance state in 3T3-L1 adipocytes through the NF-kappaB and JNK pathway.
