Study on the correlation between polymorphisms of genes with susceptibility to tuberculosis and drug-resistant tuberculosis in Chinese Han population
10.3760/cma.j.issn.0254-6450.2011.03.018
- VernacularTitle:中国汉族人群结核易感基因多态性与耐药结核病相关性研究
- Author:
Yi-Dian LIU
1
;
Rui-Juan ZHENG
;
He-Ping XIAO
;
Wei SHA
;
Qing ZHANG
;
Fu-Rong WU
;
Hua SUN
;
Zhong-Shun ZHANG
;
Hai-Yan CUI
;
Zhi-Bin LIU
;
Shen-Jie TANG
Author Information
1. 同济大学附属上海市肺科医院
- Keywords:
Pulmonary tuberculosis;
Bacterial drug resistance;
Susceptibility gene;
Polymorphism
- From:Chinese Journal of Epidemiology
2011;32(3):279-284
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution of polymorphisms of SLC11A1 gene,VDR gene,MBL gene and IFNG gene with susceptibility to tuberculosis (TB) in Chinese Han population suffering from drug-sensitive TB and drug-resistant TB so as to identify the correlation between gene polymorphisms and the development of drug-resistant TB.Methods Single nucleotide polymorphisms (SNP) of VDR gene,SLC11A1 gene,MBL gene,IFNG gene were typed and analyzed by pyrosequencing,Real-time Probe and SNaPshot among 229 patients with drug-sensitive TB and 230 patients with drug-resistant TB.Results The polymorphic foci of VDR gene from the drug-sensitive TB group and the drug-resistant TB group showed no significant difference (P＞0.05).The genotype of INT4 site and allelic frequency of SLC11A1 gene for drug-sensitive TB group were significantly different from those for drug-resistant TB group(P=0.031,0.046).If recessive inheritance was assumed,the genotypes of INT4 site from the two groups were significantly different (0R=5.756,95% CI:1.261-26.269,P=0.011).Considering the relationship between OR values under various combination,our findings confirmed that the genetic mode of INT4 site was in accordance with recessive inheritance.The genotypes of Q/P site and allelic frequencies of MBL gene from drug-sensitive and drug-resistant groups were significantly different (P=0.029,0.033).The difference still existed under the hypothesis of recessive inheritance (OR=9.290,95% CI:1.167-73.949,P=0.011).The polymorphic foci of IFNG gene from the two groups showed no significant difference.Conclusion INT4 sites on SLC11A1 gene and Q/P site on MBL gene were probably associated with the development of drug-resistant TB in Chinese Han population.Further study on this issue would be helpful in locating the population at high risk of drug-resistant TB and exploring the effective intervention to decrease the incidence of this disease.
