Mitochondrial DNA mutations---candidate biomarkers for breast cancer diagnosis in Bangladesh.
- Author:
Gazi Nurun Nahar SULTANA
1
;
Atiqur RAHMAN
;
Abu Din Ahmed SHAHINUZZAMAN
;
Rowshan Ara BEGUM
;
Chowdhury Faiz HOSSAIN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Bangladesh; Biomarkers, Tumor; metabolism; Breast Neoplasms; diagnosis; genetics; DNA Primers; genetics; DNA, Mitochondrial; genetics; Female; Humans; Middle Aged; Mutation; Odds Ratio; Polymerase Chain Reaction; Polymorphism, Genetic
- From:Chinese Journal of Cancer 2012;31(9):449-454
- CountryChina
- Language:English
- Abstract: Breast cancer is a major health problem that affects more than 24% of women in Bangladesh. Further- more, among low-income countries including Bangladesh, individuals have a high risk for developing breast cancer. This study aimed to identify candidate mitochondrial DNA (mtDNA) biomarkers for breast cancer diagnosis in Bangladeshi women to be used as a preventive approach. We screened the blood samples from 24 breast cancer patients and 20 healthy controls to detect polymorphisms in the D-loop and the ND3- and ND4-coding regions of mtDNA by direct sequencing. Among 14 distinct mutations, 10 polymorphisms were found in the D-loop, 3 were found in the ND3-coding region, and 1 was found in the ND4-coding region. The frequency of two novel polymorphisms in the D-loop, one at position 16290 (T-ins) and the other at position 16293 (A-del), was higher in breast cancer patients than in control subjects (position 16290: odds ratio = 6.011, 95% confidence interval = 1.2482 to 28.8411, P = 0.002; position 16293: odds ratio = 5.6028, 95% confidence interval = 1.4357 to 21.8925, P = 0.010). We also observed one novel mutation in the ND3-coding region at position 10316 (A > G) in 69% of breast cancer patients but not in control subjects. The study suggests that two novel polymorphisms in the D-loop may be candidate biomarkers for breast cancer diagnosis in Bangladeshi women.
