The Neuroprotective Effects of Phenytion on Ischemic-hypoxic Injury to the Developing Rat Brain.
- Author:
Young Baeg KIM
1
;
Eun Sub PARK
;
Young Hwan AHN
;
Kwan PARK
;
Byung Kook MIN
;
Sung Nam HWANG
;
Jong Sik SUK
;
Duck Young CHOI
Author Information
1. Department of Neurosurgery, College of Medicine Chung-Ang University, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Neuroprotection;
Phenytoin;
Ischemia;
Hypoxia;
Developing brain
- MeSH:
Adult;
Animals;
Anoxia;
Atrophy;
Body Temperature;
Brain Injuries;
Brain Ischemia;
Brain*;
Halothane;
Humans;
Ischemia;
Ligation;
Neurons;
Neuroprotective Agents*;
Phenytoin;
Rats*;
Rats, Sprague-Dawley;
Sodium Channels
- From:Journal of Korean Neurosurgical Society
1995;24(6):631-639
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Phenytoin is an anticonvulsant compound which modulates the voltage-dependent sodium channels. It has a neuroprotective effect in vitro against hypoxic damage in hippocampal slices of adult rats. The authors studied the efficacy of phenytion on cerebral ischemia in an vivo model of hypoxic-ischemic brain injury in developing rat brain. To elicit injury, 7 days old(P7) Sprague-dawley rats subjected to right common carotid ligation followed by 8% O2 exposure(humidified, balanced with nitrogen) for 3 hours under the halothane anesthesia(control group, N=58). Body temperature of the rats was accurately controlled before and during hypoxia. Before hypoxia, pups received intraperitoneal phenytoin(30mg/kg)(phenytoin-treated group, N=17). The animals were sacrificed one week later and histopathological evaluation of ischemic neuronal damage were conducted employing hematoxylin-eosin staining and measurement of the hemispheric weight differences were performed. Phenytoin was found to be effective in reducing neuronal damage in terms of weight comparison(24+/-2.4% atrophy of control vs. 5+/-2.9% atrophy of phenytoin group, p<0.001) and ischemic changes in hippocampal region(p<0.05 in CA1, CA2, and CA3 area). These data suggest that compounds like phenytoin, which modulates voltage-dependent sodium channels, can reduce the degree of injury from hypoxic-ischemic insults to the developing rat brain.
