Application of SCGE-FPG in the study of arsenic-induced oxidative DNA damage in PHA-stimulated and unstimulated human lymphocytes.
- Author:
Dasheng LI
1
;
Kanehisa MORIMOTO
;
Tatsuya TAKESHITA
;
Yuquan LU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Arsenic; pharmacology; DNA Damage; DNA Repair; DNA, Single-Stranded; drug effects; DNA-Formamidopyrimidine Glycosylase; Electrophoresis; methods; Humans; Hydrogen Peroxide; pharmacology; Lymphocytes; drug effects; N-Glycosyl Hydrolases; Oxidation-Reduction; Phytohemagglutinins; pharmacology
- From: Chinese Journal of Preventive Medicine 2002;36(1):12-15
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo confirm that arsenic (As) induces oxidative DNA damage in phytohemagglutinin (PHA)-stimulated and unstimulated human lymphocytes.
METHODSThe alkaline comet assay combined with specific enzyme (Formamidopyrimidine-DNA glycosylase, FPG) digestion was used to measure As-induced base damage.
RESULTSThe enzyme-sensitive sites were readily detected with the alkaline comet assay after the cells were treated with 10 micromol As for 2 hours. The repair patterns observed for FPG-created DNA single strand breaks (SSBs) in As-treated cells were comparable to those in hydrogen peroxide (H(2)O(2))-treated cells. The enzyme-created SSBs, As-induced base damage, were more significantly revealed in PHA-stimulated lymphocytes. About 63% and 68% of SSBs induced by As and H(2)O(2), respectively, were repaired in PHA-stimulated lymphocytes by 2-hour repair incubation, but about 34% and 43%, respectively, were repaired in unstimulated cells. About 40% and 49% of base damage induced by As and H(2)O(2), respectively, were repaired in PHA-stimulated lymphocytes, but about 19% and 21 %, respectively, were repaired in unstimulated cells.
CONCLUSIONSAs induces oxidative DNA damage in human lymphocytes within micromolar concentrations. Like the damage induced by H(2)O(2), As-induced DNA damage was more slowly repaired in unstimulated lymphocytes.
