The cytotoxicity of maitotoxin to LLC-PK(1) and its antagonism to calcium channel blocking agents.
- Author:
Hongying LIU
1
;
Shuangqing PENG
;
Yong SHEN
;
Weizhong SONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium; metabolism; Calcium Channel Blockers; pharmacology; Drug Antagonism; LLC-PK1 Cells; Marine Toxins; toxicity; Nifedipine; pharmacology; Oxocins; Swine; Verapamil; pharmacology
- From: Chinese Journal of Preventive Medicine 2002;36(1):22-24
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the cytotoxicity of maitotoxin (MTX) and its protective effects on calcium-channel blocking agents, so as to provide the data for control and treatment of MTX poisoning.
METHODSCytotoxicity was measured by MTT detecting system, and cytoplasmic free [Ca(2+)]i was measured by F-4500 fluorometry.
RESULTSIncubation with 8 ng/ml MTX for 3 h reduced the survival ratio of LLC-PK(1) cells. The response was found in a time- and concentration-dependent manner, with significant differences as compared with the control group. The MTX-induced increase in [Ca(2+)]i was inhibited by Verapamil and Nifedipine at 5 x 10(-5) mol/L and 1 x 10(-4) mol/L respectively. Both of them significantly reduced the death of the LLC-PK(1) cells.
CONCLUSIONSCytotoxicity of MTX may be caused by the elevated intracellular [Ca(2+)]i. Calcium-channel blocking agents could protect LLC-PK(1) cells from injury by MTX.
