The effect of vitamin C in oxidative modification of low-density lipoprotein induced by macrophage and copperion.
- Author:
Yanqiu CHEN
1
;
Qi YANG
;
Yan YANG
;
Xizhong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antioxidants; metabolism; pharmacology; Ascorbic Acid; metabolism; pharmacology; Cells, Cultured; Copper; Electrophoresis, Agar Gel; methods; Fluorescent Dyes; Lipofuscin; analysis; Lipoproteins, LDL; biosynthesis; metabolism; Macrophages, Peritoneal; cytology; drug effects; metabolism; Male; Oxidation-Reduction; Rats; Rats, Sprague-Dawley; Thiobarbituric Acid Reactive Substances; metabolism
- From: Chinese Journal of Preventive Medicine 2002;36(1):25-29
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of vitamin C on preventing oxidative modification of low density lipoprotein cholesterol (LDL) in vitro.
METHODSDifferent levels of vitamin C were added to two different systems of lipid peroxidation of LDL induced by either Cu(2+) or macrophage. The level of VC in systems induced by Cu(2+) were 10, 50, 100 and 200 micromol/L respectively and by macrophage were 50, 100, 200 micromol/L respectively. Vitamin E (200 micromol/L) and VC(0) (0 micromol/L) were served as positive and negative control respectively. The content of TBARS, Ox-LDL, fluorescent compounds, electrophoretic mobility of LDL and the lag-phage of oxidation of LDL were measured.
RESULTSIn the oxidative systems induced by Cu(2+), the groups with higher levels of VC (100, 200 micromol/L) reduced the levels of TBARS, Ox-LDL at 3, 6, and 9 hour after adding Cu(2+), but low dose groups (10, 50 micromol/L) had no the effects. In the macrophage systems, higher levels of VC (100, 200 micromol/L) significantly reduced levels of fluorescent compounds and TBARS, and also lowered electrophoretic mobility of LDL and increased the lag-phage of oxidation of LDL.
CONCLUSIONVitamin C has dual effect on oxidation of LDL. Low dose treatment enhanced oxidation of LDL, but high doses has anti-oxidative effects on LDL.
