Detection of BRAF V600E mutation in hairy cell leukemia by high- resolution melting analysis.
- Author:
Yanru ZHANG
1
;
Huimin LIU
1
;
Junyuan QI
1
;
Wei LIU
1
;
Lugui QIU
1
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; DNA Mutational Analysis; Female; Humans; Leukemia, Hairy Cell; diagnosis; genetics; Male; Middle Aged; Mutation; Polymerase Chain Reaction; methods; Proto-Oncogene Proteins B-raf; genetics
- From: Chinese Journal of Hematology 2014;35(3):207-209
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the feasibility and diagnostic implication of BRAF V600E mutation identified by high-resolution melting (HRM) assay in patients with hairy cell leukemia (HCL).
METHODSThe V600E mutation of BRAF exon 15 in four HCL patients were detected by HRM assay and patients' clinical data were retrospectively analyzed.
RESULTSAll four HCL patients were positive for the BRAF V600E mutation, which were identical to the results of DNA sequencing.
CONCLUSIONThe HRM assay for BRAF V600E mutation provides a useful tool to aid the laboratory diagnosis of HCL with easy operability, accuracy, and low cost.
