Detection and analysis of partial sequences isolated from human and swine in rural area of southern China.
- Author:
Xiao ZHANG
1
;
Ying-Jie ZHENG
;
Fa-Di WANG
;
Mei-Yang GAO
;
Jian-Fu ZHU
;
Qing-Wu JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; China; Cloning, Molecular; Feces; virology; Genes, Viral; Genotype; Hepatitis E; veterinary; virology; Hepatitis E virus; genetics; isolation & purification; Humans; Immunoglobulin M; blood; RNA, Viral; blood; Reverse Transcriptase Polymerase Chain Reaction; Rural Health; Swine; Swine Diseases; virology
- From: Chinese Journal of Epidemiology 2005;26(12):984-987
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the genotype of swines isolated from humans and their relationships with hepatitis E virus (HEV) in the rural areas of southern China.
METHODSSpecimens collected from normal people with HEV-IgM positive, acute hepatitis E patients and from swine in the same area were detected for HEV RNA using RT-nPCR with ORF2 primers. The positive PCR products were cloned and sequenced.
RESULTS13 out of the 132 samples from swine stool, 4 of 26 HEV-IgM positive sera of normal people and 1 of 4 acute hepatitis E patients' stool sample and sera were tested positive for HEV RNA. Data from sequence analysis showed that the identity at nucleotide level was 89.3%-100.0% among the 10 isolates which shared 78.7% - 84.7%, 83.3% - 85.3%, 76.0% - 80.0% and 84.7% - 95.3% nucleotide sequence identity with HEV genotype I, II, III and IV respectively in the region (nt6317- 6466).
CONCLUSIONHEV circulating in humans and swine in the area belonged to genotype IV.
