Study on the mimic epitopes screening program severe acute respiratory syndromes antigen with random phage peptide library.
- Author:
Wan-min WU
1
;
Xiao-juan WANG
;
Hui-fen ZHU
;
Guan-xin SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, Viral; Base Sequence; Biomimetic Materials; Cloning, Molecular; Enzyme-Linked Immunosorbent Assay; Epitopes; genetics; immunology; Humans; Peptide Library; Sequence Analysis, DNA; Severe Acute Respiratory Syndrome; immunology
- From: Chinese Journal of Epidemiology 2005;26(11):904-906
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen the severe acute respiratory syndromes (SARS) mimotopes with random phage peptide library and to investigate their immunogenicity.
METHODSUsing SARS sera as selective molecule, a 12 mer phage peptide library was biopanned and positive clones containing the mimic epitopes were selected. The immuno-characteriation of the epitopes were then investigated.
RESULTS2 positive clones that having specific affinity to SARS sera were obtained. The DNA sequencing data showed no homology between the sequences of the deduced amino acid of the two mimic antigen peptides and the sequence of SARS.
CONCLUSIONSARS mimotopes were obtained by phage peptide library screening. This method might provide a new approach for SARS therapy and vaccine development.
