Application of pulsed-field gel electrophoresis typing in tracing and carrying out surveillance programs on O139 cholera outbreaks.
- Author:
Hong-lu LIU
1
;
Jing-yun ZHANG
;
Ze-hui FENG
;
Wei LI
;
Zhi-gang CUI
;
Li-juan ZHANG
;
Xiao-ping ZHU
;
Biao KAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bacterial Typing Techniques; methods; China; epidemiology; Cholera; epidemiology; microbiology; transmission; Cholera Toxin; genetics; Cluster Analysis; Disease Outbreaks; Electrophoresis, Gel, Pulsed-Field; Food Microbiology; Humans; Phylogeny; Polymerase Chain Reaction; Population Surveillance; Software; Turtles; microbiology; Vibrio cholerae O139; classification; genetics; isolation & purification; Water Microbiology
- From: Chinese Journal of Epidemiology 2006;27(2):102-106
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the characteristics of molecular typing and phylogenic relationship among the Vibrio cholerae serogroup O139 strains isolated from environment and sea food samples during cholera outbreaks, in Sichuan province in 2004 and to trace the source of infections so as to support the ascertainment of epidemic control strategy.
METHODSCholera toxin gene was detected by polymerase chain reaction amplification. Pulsed-field gel electrophoresis (PFGE) was used for subtyping of isolates and clustering of patterns was analysed with the software BioNumerics.
RESULTSIn all the 72 strains under analysis, 68 appeared to be toxigenic while 4 from river water derived isolates were toxin gene negative. Sixty-seven strains were clustered into 16 PFGE patterns when digested with Not I. The patterns of toxigeinc O139 strains isolated from turtles in the markets were identical with the patterns of strains appeared in the outbreaks respectively. The PFGE patterns of isolates from different outbreaks were inconsistent.
CONCLUSIONThe sources of infection causing these outbreaks were complicated. Contaminated turtles might also be one of the major sources of outbreaks when being served at the dinner parties in Sichuan in 2004.
