Human rhinovirus detection from infants and young children with acute respiratory infections by nested-polymerase chain reaction.
- Author:
Lin-qing ZHAO
1
;
Yuan QIAN
;
Ru-nan ZHU
;
Jie DENG
;
Fang WANG
Author Information
- Publication Type:Journal Article
- MeSH: 5' Untranslated Regions; Acute Disease; Child, Preschool; Conserved Sequence; DNA, Viral; analysis; Humans; Infant; RNA, Viral; analysis; Respiratory Tract Infections; virology; Reverse Transcriptase Polymerase Chain Reaction; methods; Rhinovirus; genetics; isolation & purification; Sensitivity and Specificity
- From: Chinese Journal of Epidemiology 2006;27(2):154-156
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a rapid, sensitive and specific method for detection human rhinovirus (HRV) from clinical specimens.
METHODSPrimers derived from the highly conserved 5'noncoding region of human rhinovirus were used to develop a nested RT-PCR for detecting HRV. The sensitivity and specificity of the RT-PCR were determined using various RNA while DNA viruses were used as control. Seven hundred and seventy-one specimens collected from children with symptoms of acute respiratory infections from Nov. 2002 to Oct. 2003 were analyzed for HRV by RT-PCR as well as for other respiratory viruses through isolation of virus and indirect immunofluorescent assay.
RESULTSOnly the cDNA from HRV was positive by RT-PCR, indicating the nested RT-PCR was specific. With RT-PCR, HRV were detected in 148 out of 771 specimens (19.2%). As for HRV positive rates, it was found 53.3% in pharyngitis patients; 43.8% in laryngitis patients and 28.7% in bronchitis patients. In Sep. 2002 and from Aug. 2003 to Oct. 2003, HRV positive rates were high (21.6% - 32.6%), with Sep. 2003 in particular--32.6%. From Mar. 2003 to Jul. 2003, HRV positive rates maintained from 16.0% to 19.1%.
CONCLUSIONHRV was one of the important agents for acute respiratory infections in infants and young children in Beijing.
