Association of 1233A/G polymorphism of angiopoietin-2 gene with type 2 diabetes mellitus and diabetic nephropathy.
- VernacularTitle:2型糖尿病及其肾病与血管生成素2基因多态性的关联分析
- Author:
Quan HE
1
;
Hai-ming LUO
;
Bao-sheng ZHU
;
Xin-hua TANG
;
Lu-zhi JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Angiopoietin-2; genetics; Case-Control Studies; Chemokine CCL2; genetics; Diabetes Mellitus, Type 2; genetics; Diabetic Nephropathies; genetics; Gene Frequency; Genotype; Humans; Polymorphism, Single Nucleotide
- From: Chinese Journal of Medical Genetics 2012;29(1):72-76
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the association between single nucleotide polymorphisms (SNP) of angiopoietin-2 (Ang-2) gene and type 2 diabetes mellitus (T2DM) and diabetic nephropathy (DN).
METHODSGenotype and allele frequency of Ang-2 were detected by amplification refractory mutation system-PCR(ARMS-PCR) in 221 cases with T2DM and 104 normal controls. Monocyte chemoattractant protein-1(MCP-1) was measured by ELISA. DN patients were divided into three groups according to urinary albumin excretion rates (UAER), i.e. DN0: UAER < 30 mg/24 h, DN1: UAER 30-300 mg/24 h and DN2: UAER > 300 mg/24 h.
RESULTSStatistics showed: (1) Genotype frequencies and allele frequencies in Ang-2 1233A/G had significant difference but not Ang-2 759T/G and 1078A/G; (2) Comparing with those with genotype AA, the relative risk of genotype (AG+ GG) suffered from T2DM and DN were 2.265 fold (OR= 2.265, 95% CI: 1.223-1.402, P= 0.031), 1.789 fold (OR= 1.789, 95% CI: 0.889-1.021, P= 0.012), respectively; (3) The onset of DN was related to Ang-2 1233A/G allele G (r= 1.321, OR= 1.427, 95% CI: 2.324-4.177, P= 0.034).
CONCLUSIONAng-2 1233A/G polymorphism may be associated with T2DM and involved in onset and development of DN.
