Analysis of allele frequencies of HLA-DRB1*12:01:01G and HLA-DRB1*14:01:01G groups.

Jun-jun HE; Wei Zhang; Yan-min HE; Wei Wang; Zhe-dong Han; Nan-ying Chen; Fa-ming Zhu; Hang-jun LV; Li-xing Yan

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Analysis of allele frequencies of HLA-DRB112:01:01G and HLA-DRB114:01:01G groups.

Author: Jun-jun HE ¹ ; Wei ZHANG ; Yan-min HE ; Wei WANG ; Zhe-dong HAN ; Nan-ying CHEN ; Fa-ming ZHU ; Hang-jun LV ; Li-xing YAN
Author Information

1. Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou, Zhejiang 310006, P. R. China. zfm00@hotmail.com.
Publication Type:Journal Article
MeSH: Alleles; Exons; Gene Frequency; Genotype; HLA-DQ beta-Chains; genetics; HLA-DRB1 Chains; genetics; HLA-DRB3 Chains; genetics; Humans
From: Chinese Journal of Medical Genetics 2012;29(1):87-90
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo discriminate and analyze the relative frequencies of alleles in HLA-DRB1*12:01:01G(HLA-DRB1*12:01:01/12:06/12:10/12:17) and HLA-DRB1*14:01:01G (DRB1*14:01:01/14:54) groups and assess their associations with HLA-DRB3 and HLA-DQB1 loci.
METHODSA total of 115 DNA samples previously typed as HLA-DRB1*12:01:01G and 108 samples from HLA-DRB1*14:01:01G were selected. DNA sequences for exons 1 to 3 of the HLA-DRB1 locus were analyzed for HLA-DRB1*12:01:01G, and exons 2 to 3 were analyzed for HLA-DRB1*14:01:01G by polymerase chain reaction sequence-based typing (PCR-SBT). Genotyping of HLA-DRB3 and HLA-DQB1 were achieved by PCR-SBT.
RESULTSAmong 115 samples previously typed as HLA-DRB1*12:01:01G, 101 (87.8%) were confirmed as HLA-DRB1*12:01:01 and 14 (12.2%) were HLA-DRB1*12:10, but HLA-DRB1*12:06 and HLA-DRB1*12:17 alleles were not identified. For 108 samples previously typed as HLA-DRB1*14:01:01G, all were typed as HLA-DRB1*14:54. HLA-DRB1*12:01:01 was linked with HLA-DRB3*01:01:02 and HLA-DQB1*03:01, while HLA-DRB1*12:10 was strongly linked with HLA-DRB3*02:02:01 and HLA-DQB1*03:01. HLA-DRB1*14:54 was strongly linked with HLA-DRB3*02:02:01 and two different HLA-DQB1*05:02, *05:03 alleles.
CONCLUSIONHLA-DRB1*12:01:01 was more prevalent than HLA-DRB1*12:10 in the HLA-DRB1*12:01:01G group, and HLA-DRB1*14:54 was the dominant allele for HLA-DRB1*14:01:01G.