Expression of RAS protein in glioma and its effect on the growth of human glioma cells.
- Author:
Lei CAO
1
;
Ying-yi WANG
;
Xi-rui WANG
;
Xie-feng WANG
;
Guan SUN
;
Hui LUO
;
Ning LIU
;
Yong-ping YOU
Author Information
- Publication Type:Journal Article
- MeSH: Brain Neoplasms; genetics; metabolism; pathology; Cell Growth Processes; genetics; Cell Line, Tumor; Down-Regulation; Glioma; genetics; pathology; Humans; Immunohistochemistry; ras Proteins; biosynthesis; genetics
- From: Chinese Journal of Medical Genetics 2012;29(2):159-162
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression of RAS protein in human glioma tissues and its influence on tumor growth.
METHODSRAS protein expression in glioma tissues was determined by immunohistochemical (IHC) staining. Subsequently, MTT cell proliferation assay, flow cytometry and Western blotting were used to assay U251 cells with reduced RAS expression.
RESULTSThe expression of RAS in glioma was increased and strongly correlated with pathological grade. Downregulation of RAS resulted in glioma cells growth suppression and increased apoptosis.
CONCLUSIONThe expression level of RAS protein in human glioma was increased. Downregulation of RAS can inhibit glioblastoma cell growth through the RAS signal pathway.
