Comparison of allelic frequencies of 15 short tandem repeat loci between chronic myeloid leukemia patients and non-related healthy individuals.
- VernacularTitle:慢性髓细胞样白血病患者和正常人群短串联重复序列基因座频率的比较
- Author:
Zhi-lin WANG
1
;
Li DAI
;
Sang LI
;
Guo-qiang QIU
;
Hao-qing WU
Author Information
- Publication Type:Journal Article
- MeSH: Gene Frequency; Humans; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; genetics; Microsatellite Repeats; Polymorphism, Genetic
- From: Chinese Journal of Medical Genetics 2012;29(3):306-308
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare allelic frequencies of 15 short tandem repeat (STR) loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA) between chronic myeloid leukemia (CML) patients and non-related healthy individuals from Changzhou region in order to predict genes related with the CML.
METHODSBlood samples were collected from 745 healthy subjects and 132 CML patients with complete remission. Genotypes were determined with gene scan technology and multiplex PCR with fluorescence-labeled primers. Allelic polymorphisms of 15 STR loci were compared between the two groups. Potential genes related with CML were predicted with statistical analysis of differences in allelic frequencies.
RESULTSAllelic frequencies of 3 loci, including CSF1PO, vWA and TPOX, showed a significant difference (P<0.05) between the two groups.
CONCLUSIONCSF1PO, vWA and TPOX loci may be related with CML, albeit that the exact biologic mechanisms is unclear.
