Full sequence analysis for a null allele of MICA gene (MICA*063N).
- Author:
Ying-feng HUANG
1
;
Yin TAN
;
Shao-yu YANG
;
Hong-tu LUO
;
Tai-chen ZOU
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Base Sequence; Codon, Terminator; Exons; Female; Histocompatibility Antigens Class I; genetics; Humans; Middle Aged; Molecular Sequence Data; Sequence Analysis; methods
- From: Chinese Journal of Medical Genetics 2012;29(3):334-337
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the full nucleotide sequence of a null allele of major histocompatibility complex class I chain-related gene (MICA).
METHODSA sequence-based typing method was used to determine the nucleotide sequence of the MICA gene. Potential alleles were identified with a computer program.
RESULTSThe identified allele has possessed a sequence similar to that of MICA*027 except for a C→T substitution at position 184 in codon 62 (CAG→TAG) of exon 2. As a stop codon, this may result in a truncated protein.
CONCLUSIONA null allele of MICA gene has been identified. The sequence has been submitted to the Genbank nucleotide sequence database (submission No. HWS10011131), which was officially named as MICA*063N by the WHO Nomenclature Committee in October 2010.
