Analysis of a 452C/T single nucleotide polymorphism in γ-glutamyl hydrolase gene in children with acute leukemia.
- Author:
Hong-hong ZHANG
1
;
Li-jie YUE
;
Xiao-wen CHEN
;
Wei ZHAO
;
Chun-yan HU
;
Miao-miao ZHENG
;
Chun-lan YANG
;
Cai XIE
Author Information
- Publication Type:Journal Article
- MeSH: Acute Disease; Base Sequence; Child; Child, Preschool; Female; Gene Frequency; Genotype; Humans; Infant; Infant, Newborn; Leukemia; enzymology; genetics; Male; Polymorphism, Single Nucleotide; gamma-Glutamyl Hydrolase; genetics
- From: Chinese Journal of Medical Genetics 2012;29(3):352-355
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the distribution of γ-glutamyl hydrolase gene (GGH) 452C/T genotype and allele frequency in children with acute leukemia (AL) and healthy children.
METHODSBone marrow samples from 92 children with AL and peripheral blood samples from 124 healthy children were obtained to prepare complementary DNAs (cDNAs). The cDNAs were analyzed for a GGH 452C/T polymorphism by reverse transcriptase-polymerase chain reaction-denaturing gradient gel electrophoresis (RT-PCR-DGGE) and direct sequencing.
RESULTSThe frequencies of the AL patients with TT, CT and CC genotypes were 2.2%, 13.0% and 84.8%, and the frequencies of the control children were 1.6%, 16.9% and 81.5%, respectively. There was no significant difference in GGH genotype or T allele frequency between the two groups (P> 0.05). However, the T allele frequency in Han Chinese children was significantly different from those reported in Japanese, Mexican and African-American populations.
CONCLUSIONThe frequency of 452C/T polymorphism of GGH gene in Han Chinese children has been determined. The results suggested that an ethnic difference may exist.
