Theoretical and experimental studies on solubility parameters of multiple components for traditional Chinese materia medica with HPLC.
- Author:
Fu-Yuan HE
1
;
Hong-Hao ZHOU
;
Jie-Ying LUO
;
Kai-Wen DENG
;
Jin FU
;
Feng-Juan PI
;
De-Zhi WU
;
Ru-Wen DAI
Author Information
- Publication Type:Journal Article
- MeSH: Anthraquinones; chemistry; Caffeine; chemistry; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; chemistry; Emodin; analogs & derivatives; chemistry; Materia Medica; chemistry; Models, Chemical; Regression Analysis; Solubility; Temperature
- From: China Journal of Chinese Materia Medica 2008;33(6):642-648
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the theories and methods to determine solubility parameters of multiple components for the traditional Chinese material medica (TCMM) with HPLC.
METHODThe mathematical expresses to determin the solubility parameters were established according to chromatographic and Hildebrand-Scatchard theories, The HPLC experiments were carried out at 40 degrees C on an Alltech Apollo C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetone and water in gradient mode. The flow rate was 1.0 mL min(-1), and the detection wavelength was 254 nm. The injection volume was 1 0 microL.
RESULTThe mathematical expresses between the retention time and the solubility parameters were established and used to determin caffeine solubility parameter as 28.31 J(1/2) cm(-3/2) in accordance with 28.84 J(1/2) cm(-3/2) reported by literature, and those of aloe-emodin, rhein, emodin, physcione as 39.70 J(1/2) cm(-3/2), 39.08 J(1/2) cm(-3/2), 38.37 J(1/2) cm(-3/2), 36.42 J(1/2) cm(-3/2) respectively.
CONCLUSIONThe retention time of HPLC can be used to determine the solubility parameters of multiple componets. The established method is useful for the compatibility rule study of traditional Chinese medicine.
