Determination of 1, 2, 3, 4, 6-penta-O-galloyl-D-glucose in forty four kinds of Chinese traditional medicines by HPLC.
- Author:
Wei-Cong WANG
1
;
Chao WANG
;
Xue-Ying SONG
;
Wen-Hua ZHAO
;
Qiao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Calibration; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; chemistry; Hydrolyzable Tannins; analysis; Sensitivity and Specificity
- From: China Journal of Chinese Materia Medica 2008;33(6):656-659
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine 1, 2, 3, 4, 6-penta-O-galloyl-D-glucose (PGG) in forty four kinds of Chinese traditional medicines by HPLC.
METHODThe PGG was extracted with a solvent consisting of ethanol and water (50:50) in ultrasonic bath at 50-60 degrees C for 1 hour. A Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) was used for the separation and analysis of PGG with a mobile phase consisting of acetonitrile and 2. 5 % acetic acid solution (18:82). The wavelength of detection was at 280 nm, and flow rate was set at 1.0 mL min(-1).
RESULTThe calibration curve for PGG is linear over the range of 1-150 microg mL(-1) (r =0.9994), and PGG was found in seventeen kinds of Chinese traditional medicines, such as gall tree peony bark, red peony root, white peony root, and so on.
CONCLUSIONThe contents of PGG were determined in forty four kinds of Chinese traditional medicines by a rapid and precision HPLC method.
