Protective effects of Guangdong Liangcha grandes on restraint stress-induced liver damage in mice.
- Author:
Li BAO
1
;
Xin-Sheng YAO
;
Rong-Rong HE
;
Hiroshi KURIHARA
Author Information
- Publication Type:Journal Article
- MeSH: Alanine Transaminase; blood; Animals; Drugs, Chinese Herbal; chemistry; pharmacology; Glutathione; metabolism; Liver; drug effects; metabolism; pathology; Liver Diseases; blood; etiology; metabolism; prevention & control; Male; Mice; Mice, Inbred C57BL; Nitric Oxide; metabolism; Oxidative Stress; drug effects; Restraint, Physical; Stress, Psychological; complications
- From: China Journal of Chinese Materia Medica 2008;33(6):664-669
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Guangdong Liangcha Keli on restraint stress-induced liver damage in mice.
METHODThirty-five male C57BL/6J mice of 7 weeks old were divided into 5 groups randomly with 7 mice in each group: normal group, restraint stress group, 250 mg kg(-1) Vitamin C, Guangdong Liangcha Keli 500 mg kg(-1) and 250 mg kg(-1). After 18 hr restraint stress, the ALT acitivity in plasma, MDA level in plasma and liver, GSH content, GSH-PX and GST activities, NO level and ORAC value in liver were determined.
RESULTCompared with restraint model group, Guangdong Liangcha Keli could markedly reduce ALT activity (92.75 +/- 1.91 vs 39.29 +/- 2.56, 32.69 +/- 1.46) U L(-1), and protect the liver damage induced by oxidative stress. In addition, Guangdong Liangcha Keli could effectively increase the ORAC value, GSH content, GSH-PX activity and GST activity and reduce the MDA level and NO level in liver.
CONCLUSIONOral treatment of Guangdong Liangcha Keli is found to reduce restraint stress-induced liver damage in terms of above mentioned biochemical parameters, and these protective effects may be related to its free radical scavenging activity and lipid peroxidation inhibitory effects.
