Effect of total glucosides of paeony on nuclear factor-kappaB activation in rat peritoneal macrophages.
- Author:
Gang CHEN
1
;
Xiao-Hong DENG
;
Li-Xia GUO
;
Jian-Hui LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Nucleus; drug effects; metabolism; Cytoplasm; drug effects; metabolism; DNA; metabolism; Dose-Response Relationship, Drug; Glucosides; pharmacology; Macrophages, Peritoneal; cytology; drug effects; metabolism; NF-kappa B; metabolism; Paeonia; chemistry; Protein Transport; drug effects; Rats; Transcription Factor RelA; metabolism
- From: China Journal of Chinese Materia Medica 2008;33(6):669-671
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of total glucosides of paeony (TGP) on lipopolysaccharides (LPS)-induced nuclear factor-kappaB (NF-kappaB) activation in macrophages.
METHODRat peritoneal macrophages were pre-treated with TGP for 2 h and stimulated with LPS for 20 min or 0.5 h. Inhibitory kappaBalpha (IkappaBalpha) protein in the cytoplasm and NF-kappaB p65 protein in the nuclear were analyzed by western blot. Further, DNA binding activity of NF-kappaB complex was detected.
RESULTTGP enhanced the amounts of IkappaBalpha protein in the cytoplasm and decreased the amounts of NF-kappaB p65 protein in the nuclear of LPS-induced macrophages. TGP also inhibited the LPS-mediated DNA binding activity of NF-kappaB complex in macrophages.
CONCLUSIONTGP can inhibit LPS-induced NF-kappaB activation in macrophages through arresting IKBalpha protein degradation, NF-kappaB p65 protein nuclear translocation and DNA binding activity of NF-kappaB complex.
