Protective mechanism on the vascular pathological process in diabetes mellitus rats by Rheum officeinale.
- Author:
Feng-Sheng TIAN
1
;
Zhen-Bin LI
;
Yuan-Song WANG
;
Xiu-Hai SU
;
Wen-Dong LI
;
Xiao-Yun WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aorta; drug effects; metabolism; pathology; Blood Glucose; metabolism; Blood Vessels; drug effects; metabolism; pathology; Diabetes Mellitus; pathology; Drugs, Chinese Herbal; pharmacology; Endothelin-1; metabolism; Endothelium, Vascular; drug effects; metabolism; pathology; Gene Expression Regulation; drug effects; Intercellular Adhesion Molecule-1; metabolism; Male; Nitric Oxide; metabolism; Protective Agents; pharmacology; Rats; Rheum; chemistry
- From: China Journal of Chinese Materia Medica 2008;33(6):672-675
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the protective mechanism of officeihale on the vascular pathological process in diabetes mellitus (DM) rats.
METHODAfter the DM rat model was established, 24 DM rats were randomly divided into model group (12 DM rats) and Rheum officeinale group (12 DM rats). Rheum officeinale was orally given in 10 g kg(-1) per day, and the other two groups were given equal pure water. 8 weeks later, blood samples were collected to determine the level of nitric oxide (NO) and endothelin-1 (ET-1). Thoracic aortic rings was prepared to observe the inhibiting effect of Ach with different concentration on contraction caused by NE. Another part of aorta was made to observe the expression of ICAM-1 and VCAM-1 by method of SP immunohistochemistry staining,
RESULTRheum officeinale group obviously decreased the level of ET-1 and increased the NO compared with model group (P <0.05). The expression of ICAM-1 and VCAM-1 could be obviously inhibited in Rheum officeinale group compared with model group. (P <0.05).
CONCLUSIONRheum officeinale could decrease the level of ET-1 with increased the NO in diabetes rats, and inhibit the expression of ICAM-1 and VCAM-1, which may be mechanisms of protecting the endothelium of vessel in diabetes rats.
