Effects of rat serum containing total flavonoid extract of Epimedium sagittatum on development of osteoblasts.
- Author:
Hui-Ping MA
1
;
Zheng-Ping JIA
;
Ke-Ming CHENG
;
Ru-Xue ZHANG
;
Mao-Xing LI
;
Juan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcification, Physiologic; drug effects; Calcium; metabolism; Cell Differentiation; drug effects; Cell Proliferation; drug effects; Drugs, Chinese Herbal; pharmacology; Epimedium; chemistry; Flavonoids; blood; pharmacology; Osteoblasts; cytology; drug effects; metabolism; Rats; Rats, Wistar
- From: China Journal of Chinese Materia Medica 2008;33(8):928-931
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of total flavonoid extract of Epimedium sagittatum (TFE) on the proliferation and differentiation of newborn rat calvarial osteoblasts (ROB).
METHODTFE was supplemented into the culture medium of ROB at 0. 1, 1, 10 and 100 microg x mL(-1) respectively. The serum of rats administered TFES (SRAT) was also added into the medium in a parallel treatment at 2.5%, 5% and 10% respectively. Their effects on cell proliferation and differentiation was studied by MTT and the analysis of osteogenic differentiation marks.
RESULTTFE had no appreciable and on cell proliferation and differentiation at any concentration. However, 2.5% and 5% SRAT stimulated cell proliferation strongly and, 5% SRAT significantly promoted the maturation and function of osteoblast by improving the alkaline phosphatase activity, osteocalcin secretion, calcium deposition and the number of mineralized nodular structures.
CONCLUSIONThe metabolites of TFE should be the anti-osteoporosis constitutes of Epimedium sagittatum.
