Biotransformation of podophyllotoxin by cell suspension culture and root culture of Rheum palmatum.
- Author:
Ya-Jun CUI
1
;
Xiao-Feng LIU
;
Jian HAN
;
Bao-Rong WANG
;
De-An GUO
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Molecular Structure; Plant Roots; metabolism; Podophyllotoxin; chemistry; metabolism; Rheum; metabolism; Tissue Culture Techniques; methods
- From: China Journal of Chinese Materia Medica 2008;33(9):989-991
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the biotransformation of podophyllotoxin by the cell suspension culture and root culture systems of Rheum palmatum.
METHODUsing plant tissue culture technology and HPLC techniques to isolate products. The structures were elucidated by spectroscopic means.
RESULTCell suspension culture of R. palmatum could convert podophyllotoxin to produce picropodophyllotoxin with the yield of 73.8%, while root culture of R. palmatum could convert podophyllotoxin to produce epipodophyllotoxin and apopodophyllotoxin.
CONCLUSIONPodophyllotoxin did not affect the pH value of the media used in tissue cultures. Both cell suspension culture and root culture of R. palmatum can convert podophyllotoxin.
