Expression of NR1 mRNA of NMDA receptor by gastrodine on hypoxia injury in cultured rat cerebral cortical neurons.
- Author:
Lei FU
1
;
Yan-Hua MAO
;
Yuan GAO
;
Lei LIU
;
Zhi-Ping WANG
;
Liang-Chen LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Benzyl Alcohols; pharmacology; Cells, Cultured; Cerebral Cortex; cytology; Female; Gene Expression Regulation; drug effects; Glucosides; pharmacology; Hypoxia; genetics; Neurons; cytology; metabolism; Pregnancy; RNA, Messenger; genetics; Rats; Rats, Wistar; Receptors, N-Methyl-D-Aspartate; genetics; Reverse Transcriptase Polymerase Chain Reaction
- From: China Journal of Chinese Materia Medica 2008;33(9):1049-1052
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of gastrodine on the expression of NR1 mRNA of NMDA receptor in cultured rat cerebral cortical neurons injury induced by hypoxia.
METHODThe injury models of cultured rat cerebral cortical neurons was made by hypoxia and hypoglucose. The concentration of 13, 26, 52 mg x L(-1) of gastrodine was respectively added to the injured cells. The R-PCR technique was used to study the expression of NR1 mRNA of NMDA receptor.
RESULTThe expression of NR1 mRNA increased markedly in hypoxia and hypoglucose injured cells, the increased expression can be weakened by gastrodine, more significant effect was found in the concentration of 26 mg x L(-1) 52 mg x L(-1).
CONCLUSIONGastrodine may act as a neuro - protector by weakening the expression of NR1 mRNA in cultured rat cerebral cortical neurons injury induced by hypoxia and hypoglucose.
