Effects of Chinese herb compound Naoyikang on expression of choline acetyltransferase in brain of rats with Alzheimer's disease.
- Author:
Jin-Song GENG
1
;
Ai-Ling ZHOU
;
Hai-Yan SHI
;
Ya-Er HU
;
Jia-Hui MAO
;
Yan ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Alzheimer Disease; enzymology; Animals; Blotting, Western; Brain; drug effects; enzymology; Choline O-Acetyltransferase; metabolism; Disease Models, Animal; Drugs, Chinese Herbal; pharmacology; Gene Expression Regulation; drug effects; Immunohistochemistry; Male; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2008;33(9):1071-1074
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of Naoyikang (NYK) on expression of choline acetyltransferase (ChAT) in brain of rats with Alzheimer' s disease (AD).
METHODBilateral infusions of Ibotenic acid (IBO) into nucleus basalis of Meynert (NBM) using hamilton syringe and stereotaxic apparatus were adopted to establish the rat model of AD. After intragastrically administrated with different solution for 28 days, immunohistochemistry and Western-blot were adopted to study the expression of ChAT in frontal cortex of AD rats.
RESULTNYK could improve the morphology and increase the number of ChAT immunoreactive neurons, and significantly promote ChAT protein expression.
CONCLUSIONNYK may be able to increase the synthesis of acetylcholine (ACh) through elevating the expression of ChAT protein, thus improving the level of brain ACh so as to protect central cholinergic neurons.
