Genetic diversity evaluation of ex-situ conservation population of Phellodendron amurense detected by AFLP markers.
- Author:
Zhi-Feng YAN
1
;
Ben-Gang ZHANG
;
Zhao ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Amplified Fragment Length Polymorphism Analysis; Conservation of Natural Resources; DNA Primers; genetics; Genetic Variation; Phellodendron; classification; genetics; Phylogeny; Polymorphism, Genetic
- From: China Journal of Chinese Materia Medica 2008;33(10):1121-1125
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the genetic diversity from ex-situ conservation population of Phellodendron amurense.
METHODGenetic diversity in 67 individuals from ex-situ conservation population of P. amurense were assessed using AFLP technique by eight fluorescent labeled primer groups.
RESULTThe eight primer combinations generated a total of 1,574 bands, of which 1,354 were polymorphic. As for P. amurense at species level, the percentage of polymorphic loci (PPL) was 86.02, the effective number of alleles per locus (Ne) was 1.4275 ,the Nei's gene diversity (H) was 0.2312, and the Shannon's information index (I) was 0.3973. At the population level, the estimates PPL=41.55, H=0.1400, Ne=0.2376 and I=0.2113.
CONCLUSIONThe effect of present ex-situ conservation was preliminarily assessed.
