Separation and purification process of total flavones and total tannins from Apocynum venetum leaves with macroreticular resin.
- Author:
Su-Qiong ZHANG
1
;
Jin-Jun HOU
;
Qing-Shan LI
Author Information
- Publication Type:Journal Article
- MeSH: Adsorption; Apocynum; chemistry; Chromatography, Liquid; methods; Drugs, Chinese Herbal; chemistry; isolation & purification; Flavones; chemistry; isolation & purification; Plant Leaves; chemistry; Resins, Synthetic; chemistry; Tannins; chemistry; isolation & purification
- From: China Journal of Chinese Materia Medica 2008;33(10):1141-1230
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the purification process of total flavones and total tannins from Apocynum venetum leaves with macroreticular resin.
METHODThe static capacity absorption, dynamic absorption ratio and dynamic elution ratio of different types of macroreticular resins were studied and compared in order to find the best one among the eight macroreticular resins, and the technical process of the type of HPD-400 type macroreticular resin was studied in detail.
RESULTThe type of HPD-400 type macroreticular resin showed the best comprehensive absorption property with the following technological conditions: the current velocity was 1 BV x h(-1), the volume of distilled water was 2 BV, the eluting reagent was 60% ethanol, and the volume of 60% ethanol was 3 BV.
CONCLUSIONThe purity of total flavones and total tannins in the product is up to 80% after purified with HPD-400 macroreticular resin. Therefore, this purification process is feasible.
