Induction of Nestin Early Expression as a Hallmark for Mesenchymal Stem Cells Expression of PDX-1 as a Pre-disposing Factor for Their Conversion into Insulin Producing Cells.
- Author:
Marisela MARTINEZ-GAMBOA
1
;
Delia Elba CRUZ-VEGA
;
Jorge MORENO-CUEVAS
;
Maria Teresa GONZALEZ-GARZA
Author Information
- Publication Type:Original Article
- Keywords: Mesenchymal stem cells; PDX-1; Insulin producing cells; Nestin
- MeSH: Cell Culture Techniques; Cell- and Tissue-Based Therapy; Ethnic Groups; Glucose; Humans; Insulin*; Mesenchymal Stromal Cells*; Methods; Nestin*
- From:International Journal of Stem Cells 2017;10(1):76-82
- CountryRepublic of Korea
- Language:English
- Abstract: Diabetes constitutes a worldwide epidemic that affects all ethnic groups. Cell therapy is one of the best alternatives of treatment, by providing an effective way to regenerate insulin-producing cells lost during the course of the disease, but many issues remain to be solved. Several groups have been working in the development of a protocol capable of differentiating Mesenchymal Stem Cells (MSCs) into physiologically sound Insulin Producing Cells (IPCs). In order to obtain a simple, fast and direct method, we propose in this manuscript the induction of MSCs to express NESTIN in a short time period (2 h), proceeded by incubation in a low glucose induced medium (24 h) and lastly by incubation in a high glucose medium. Samples from cell cultures incubated in high glucose medium from 12 to 168 h were obtained to detect the expression of INSULIN-1, INSULIN -2, PDX-1 and GLUT-2 genes. Induced cells were exposed to a glucose challenge, in order to assess the production of insulin. This method allowed us to obtain cells expressing PDX-1, which resembles a progenitor insulin-producing cell.
