Gene transfer of CD151 enhanced myocardial angiogenesis and improved cardiac function in rats with experimental myocardial infarction.
- Author:
Li WANG
1
;
Jun YANG
;
Zheng-xiang LIU
;
Bin CHEN
;
Jian LIU
;
Rong-fang LAN
;
Jin QIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, CD; genetics; Disease Models, Animal; Genetic Therapy; Genetic Vectors; Male; Myocardial Infarction; physiopathology; therapy; Neovascularization, Physiologic; Rats; Rats, Sprague-Dawley; Tetraspanin 24; Transfection; Ventricular Function, Left
- From: Chinese Journal of Cardiology 2006;34(2):159-163
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of in vivo CD151 gene transfer on angiogenesis and heart function in rats with myocardial infarction.
METHODSAcute myocardial infarction (AMI) was induced in male Sprague-Dawley (SD) rats by left anterior descending coronary artery ligation. The surviving rats randomly received myocardial injection of saline (MI control), pAAV-CD151 and pAAV-GFP (n = 12/group). Sham-operated rats without myocardial injection (n = 12) were taken as normal control. Four weeks later, heart function and the expression of CD151 were measured. Micro vessels density (MVD) in infarct myocardium was observed by factor VIII related antigen immunochemical staining.
RESULTSThe expression of CD151 (1.98 +/- 0.23 vs. 0.91 +/- 0.09, P < 0.01) and MVD counting [(385.4 +/- 79.9) vs. (252.5 +/- 43.0) n/mm(2), P < 0.01] in pAAV-CD151 treated MI rats were significantly higher than that in MI control group, similarly, EF (64.0 +/- 8.7)% vs. (41.5 +/- 5.0)%, P < 0.01] and dp/dt(max) (6620.2 +/- 884.6 vs. 5545.5 +/- 693.0, P < 0.01) were also significantly increased post pAAV-CD151 treatment. These parameters were not affected by pAAV-GFP treatment.
CONCLUSIONCD151 in vivo gene transfer for rats with acute myocardial infarction enhanced myocardial angiogenesis and improved left ventricular function.
