Effects of rapamycin on Rho-kinase and p27 mRNA expressions in a porcine coronary intimal proliferation model induced by interleukin-1beta.
- Author:
Zhi-lin MIAO
1
;
Ding-yin ZENG
;
Xi-zhuo SUN
;
Xu-chen ZHOU
;
Ying CHENG
;
Qi-gang GUAN
;
Li ZHANG
;
Xue-zhi HE
;
Feng-tong HAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Coronary Angiography; Coronary Vessels; drug effects; metabolism; pathology; Disease Models, Animal; Interleukin-1beta; pharmacology; Male; RNA, Messenger; metabolism; Sirolimus; pharmacology; Swine; Tunica Intima; drug effects; metabolism; pathology; rho-Associated Kinases; metabolism
- From: Chinese Journal of Cardiology 2006;34(5):445-449
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of rapamycin on the expressions of Rho-kinase and p27 mRNA during vascular intimal proliferation in a porcine model of coronary stenosis induced by interleukin-1beta (IL-1beta).
METHODSThe proximal segments of LAD and LCX were wrapped with cotton mesh that had absorbed sepharose bead solution with or without IL-1beta. Selective coronary angiography was performed two weeks later and the animals were killed for collecting the samples for histopathology and RT-PCR analyzing of Rho-kinase and p27 mRNA.
RESULTSThe expressions of Rho-kinase and p27 mRNA could be visualized in normal coronary wall. The expression of Rho-kinase mRNA was significantly enhanced and the expression of p27 mRNA was significantly decreased during the process of intimal proliferation induced by IL-1beta. Rapamycin significantly inhibited the intimal proliferation, reduced the infiltration of inflammatory cells, reduced the expression of Rho-kinase mRNA and increased the expression of p27 mRNA.
CONCLUSIONSThe expression of Rho-kinase mRNA is upregulated and p27 mRNA downregulated in coronary artery stenosis induced by IL-1beta and these effects could be abolished by cotreatment with rapamycin.
