PPARgamma1 overexpression on caveolin-1 expression of Raw264.7 cells.
- Author:
Qin HU
1
;
Yun ZHANG
;
Chun-xi LIU
;
Mei ZHANG
;
Ma JING
;
Hong HE
;
Jin-bo FENG
;
Rong WANG
;
Gui-hua JIANG
;
Xian-jun ZHANG
;
Hong JIANG
;
Qing ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Animals; Caveolin 1; metabolism; Cell Line; Chromans; pharmacology; Gene Expression; Macrophages; drug effects; metabolism; Mice; PPAR gamma; genetics; RNA, Messenger; metabolism; Thiazolidinediones; pharmacology
- From: Chinese Journal of Cardiology 2006;34(5):458-463
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of PPARgamma1 gene overexpression on caveolin-1 mRNA and protein expressions in a murine macrophage cell line Raw264.7.
METHODSReplication-deficient recombinant adenovirus expression vector of PPARgamma1 was constructed using the AdEasy system. Raw264.7 cells were randomly treated as follows: P group (PPARgamma1 gene overexpression), T group (Troglitazone 40 micromol/L in DMSO), PT group (PPARgamma1 gene overexpression and Troglitazone) and control group. Changes of PPARgamma1 and caveolin-1 at mRNA and protein levels were investigated.
RESULTSCaveolin-1 expression can be detected by RT-PCR in Raw264.7, by immunocytochemistry method in cell and nuclear membrane but not by immunoblotting at protein level. Caveolin-1 expression at mRNA and protein levels in Raw264.7 were significantly higher in P, T and PT groups compared to control group and the expression was also significantly higher in PT group than that in P group and T group (P < 0.05). PPARgamma expression was significantly increased in PT group and P group where remained unchanged in T group compared to control group.
CONCLUSIONPPARgamma1 overexpression can upregulate caveolin-1 expression in macrophages. Troglitazone upregulated caveolin-1 expression in the absence of increased PPARgamma1 expressions at mRNA and protein levels.