Immunological killing effect of recombicant adenovirus vector rAD-mTERT-m4-1BBL on mouse hepatoma cell line Hepa1-6 cells co-cultured with T lymphocytes.
- Author:
Zhang-sheng XIAO
1
;
Shi-yin MA
;
Wei-da GONG
;
Hui-hua YAO
;
Peng DU
;
Ying-qing XING
;
Hao-rong WU
Author Information
- Publication Type:Journal Article
- MeSH: 4-1BB Ligand; genetics; physiology; Adenoviridae; genetics; Animals; Apoptosis; CD4-CD8 Ratio; Cell Line; Cell Line, Tumor; Cell Proliferation; Coculture Techniques; Fibroblasts; cytology; Genetic Vectors; Liver Neoplasms, Experimental; immunology; pathology; Mice; Mice, Inbred C57BL; Promoter Regions, Genetic; Recombinant Proteins; genetics; T-Lymphocytes; immunology; Telomerase; genetics; Transfection
- From: Chinese Journal of Oncology 2009;31(12):894-898
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the immunological suppressing effect of recombinant adenovirus vector rAD-mTERT promotor-m4-1BBL (rAD-mTERT) on mouse hepatoma cell line Hepa1-6 cells in co-culture with T lymphocytes.
METHODSAdding recombinant adenovirus rAD, rAD-CMV-m4-1BBL (rAD-CMV) and rAD-mTERT to Hepa1-6 and L929 cells, respectively, to observe the effect of these adenoviruses on growth and apoptosis of these cells in co-culture with T lymphocytes.
RESULTSAdding adenovirus significantly suppressed the growth and slightly increased apoptosis of the two types of cells (P < 0.05). rAD-mTERT promotor-m4-1BBL showed only pro-apoptotic effect on Hepa1-6 cells. When co-cultured with T lymphocytes, rAD-CMV-m4-1BBL showed promoting effect on apoptosis of the cells. Compared with that of T cells pre-co-culture, CD4(+) and CD8(+) T cells were proliferated, and the ratio of CD4/CD8 was significantly reduced (from 1.27 to 1.08).
CONCLUSIONAdding the recombinant adenoviruses only suppresses the cell growth, but not promotes their apoptosis. In co-culture with T lymphocytes, recombinant adenovirus vector rAD-mTERT promotor-m4-1BBL can targetingly suppress the growth and induce apoptosis of Hepa1-6 cells. The apoptosis is induced through the immunological killing effect of T lymphocytes.
