Expression of human papillomavirus type 16/18 in human cervical carcinomas by the quantum dot fluorescent in-situ hybridization.
- Author:
Jun SUN
1
;
Jun-bo HU
;
Hong-lei CHEN
;
Bei-yun LI
;
He-shun XIA
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Carcinoma, Squamous Cell; pathology; virology; Cervical Intraepithelial Neoplasia; pathology; virology; Chromogenic Compounds; Female; Human papillomavirus 16; isolation & purification; Human papillomavirus 18; isolation & purification; Humans; In Situ Hybridization, Fluorescence; methods; Middle Aged; Papillomavirus Infections; virology; Quantum Dots; Sensitivity and Specificity; Uterine Cervical Neoplasms; pathology; virology
- From: Chinese Journal of Pathology 2010;39(10):675-677
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate fluorescence in situ hybridization labeled with quantum dots (QDs) for the detection of human papillomavirus 16/18 (HPV16/18) infection in cervical carcinoma patients.
METHODSA total of 80 biopsy samples of squamous carcinoma of cervix were assayed for HPV 16/18 infection by using quantum dot labeled fluorescent in situ hybridization (QD-FISH) and chromogenic in situ hybridization (CISH) techniques, respectively. The results obtained by using two different methods were statistically analyzed.
RESULTSThe positive rate for HPV16/18 by QD-FISH was 88.8% (71/80), higher than that (80.0%) by CISH, however, the result was statistically not significant (P=0.127). The positive detection rates for HPV16/18 by using both methods increased coincidentally with raising of the tumor grading stage.
CONCLUSIONThe sensitivity and specificity of HPV infection detectable by QD-FISH is higher than that by the CISH technique.
