Analysis of chromosomal abnormalities in pancreatic cancer by spectral karyotyping.
- Author:
Zhi-Mei YANG
1
;
Xiao-Ping HAN
;
Sha-Fei WU
;
Yu-Feng YIN
;
Ke WANG
;
Jie GAO
;
Zhi-Yong LIANG
;
Xuan ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; genetics; Aged; Cell Line, Tumor; Chromosome Aberrations; Chromosome Deletion; Chromosome Duplication; Female; Gene Dosage; Genes, erbB-1; genetics; Humans; In Situ Hybridization, Fluorescence; Karyotyping; methods; Male; Middle Aged; Pancreatic Neoplasms; genetics
- From: Chinese Journal of Pathology 2010;39(11):767-771
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEto investigate the chromosomal characteristics of pancreatic ductal adenocarcinomas by spectral karyotyping.
METHODScytogenetic aberrations of pancreatic cancer cell line P2 established from a Chinese patient was investigated by spectral karyotyping (SKY). Chromosomal alterations were further evaluated in 10 cases of pancreatic cancer and 10 cases of chronic pancreatitis by two color fluorescence in situ hybridization (FISH) by using EGFR/CEP7 probe and paraffin embedded tissue samples.
RESULTShypotriploid and 26 chromosomal aberrations were revealed in cell line P2. Recurrent chromosomal numerical alterations included loss of chromosome 4, 9, 18, 19, 22, Y, 10p, 15p, 8p, 6q and 12p, with gain of chromosome 7 and 12q. Frequent chromosomal structural abnormalities included der(9;15)(q10;q10), der(10)(3;10)(?;q26) and der(12)(8;12)(?;p13). Four of 10 cases showed EGFR copy number changes by FISH.
CONCLUSIONShighly complex chromosomal rearrangements occur in pancreatic cancers. Additional studies of more cases are needed, including FISH analysis of EGFR copy number changes, to reach a conclusion.
