Expression of COX-2 in different human prostate cancer cell lines and its significance.
- Author:
Jia-Hui ZHAO
1
;
Yong-Guang JIANG
;
Chun-Ting WU
;
Yong LUO
;
Zhu HOU
;
Da-Lin HE
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cyclooxygenase 2; metabolism; Humans; Male; Neoplasm Metastasis; Prostatic Neoplasms; metabolism; pathology; RNA, Messenger; genetics
- From: National Journal of Andrology 2010;16(8):689-692
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of cyclooxygenase-2 (COX-2) in different prostate cancer (PCa) cell lines and its role in the acquisition of invasive and metastatic potentials of PCa.
METHODSWe detected the expressions of COX-2 in prostate cancer cell lines LNCaP, C4-2, IF11, IA8 and PC-3 with different metastatic potentials by Western blotting and RT-PCR, and analyzed their roles in the invasion and metastasis of different PCa cell lines.
RESULTSWestern blotting and RT-PCR showed that the expression of the COX-2 protein was high in PC-3, but absent in IF11, IA8, LNCaP and C4-2 (P < 0.05), and it was consistent with the expression of COX-2 mRNA.
CONCLUSIONCOX-2 expresses differently in PCa cell lines with different metastatic potentials. The overexpression of COX-2 may be associated with the high invasion and metastasis of PC-3, but not with the metastasis of other cell lines.
