Application of iTRAQ in proteomic study of prostate cancer.
- Author:
Chuan-Yu SUN
1
;
Guo-Wei XIA
;
Ke XU
;
Qiang DING
Author Information
1. Department of Urology, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, China. drsunchuanyu@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Humans;
Isotope Labeling;
methods;
Male;
Prostatic Neoplasms;
metabolism;
Proteomics;
methods
- From:
National Journal of Andrology
2010;16(8):741-744
- CountryChina
- Language:Chinese
-
Abstract:
Amine-specific isobaric tagging (iTRAQ) reagents, as a new class of isobaric reagent, were developed in 2004, which, combined with liquid chromatography and tandem mass spectrometry (LC-MS/MS) , have been applied to the identification and quantification of proteins in a wide range of biological samples, including bacteria, yeasts, human tissues, cells, and fluids. As a new method of quantitative proteomics, the technique of iTRAQ allows for the quantitative analysis of four samples simultaneously and displays its advantages of high-flux, food reproducibility, and high sensitivity; it also provides a potential technological platform for studying the mechanisms of the development and progression of prostate cancer.
