Comparison of the inhibitory activities of salvianolic acid B and Ginkgo biloba extract EGb 761 on neurotoxicity of beta-amyloid peptide.
- Author:
Chang-suo LIU
1
;
Jin-feng HU
;
Nai-hong CHEN
;
Jun-tian ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Amyloid beta-Peptides; chemistry; toxicity; ultrastructure; Animals; Apoptosis; drug effects; Benzofurans; isolation & purification; pharmacology; Cell Survival; drug effects; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; isolation & purification; pharmacology; Flow Cytometry; Ginkgo biloba; chemistry; Intracellular Fluid; drug effects; metabolism; Microscopy, Electron; Neuroprotective Agents; isolation & purification; pharmacology; PC12 Cells; Peptide Fragments; chemistry; toxicity; ultrastructure; Plant Extracts; isolation & purification; pharmacology; Plants, Medicinal; chemistry; Rats; Reactive Oxygen Species; metabolism; Salvia miltiorrhiza; chemistry
- From: Acta Pharmaceutica Sinica 2006;41(8):706-711
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo compare the effects of salvianolic acid B (Sal B) and Ginkgo biloba extract EGb 761 on beta-amyloid peptide (beta-AP) fibril formation and cytotoxicity to PC12 cells.
METHODSThe inhibitory effects of Sal B and EGb 761 on beta-AP1-40 fibril formation were determined by using fluorescence analysis with Thioflavin T (ThT) and electron microscopic image. beta-AP25-35 was aged by incubating at 37 degrees C for 7 d, then the protein was incubated with PC12 cells. The protective effects of Sal B and EGb 761 against cytotoxicity induced by aged beta-AP25-35 in PC12 cells were evaluated by MTT reduction assay and flow cytometric analysis. beta-AP25-35-induced accumulation of intracellular reactive oxygen species (ROS) was determined by fluorescence analysis.
RESULTSBoth Sal B and EGb 761 inhibited the formation of amyloid fibrils, protected PC12 cells from beta-AP25-35-induced cytotoxicity, and decreased ROS accumulation caused by beta-AP25-35. The effective doses of Sal B were far lower than those of EGb 761.
CONCLUSIONSal B was much more efficient than EGb 761 in inhibiting beta-AP aggregation and in protecting PC12 cells from beta-AP-induced cytotoxicity.
