RP-HPLC fingerprint for quality assessment of Radix Angelicae dahuricae.
- Author:
Ting-ting WANG
1
;
Xiao-hui CHEN
;
Qing-qing HU
;
Kai-shun BI
Author Information
- Publication Type:Journal Article
- MeSH: Angelica; chemistry; Chromatography, High Pressure Liquid; methods; Cluster Analysis; Plant Extracts; analysis; chemistry; standards; Plant Roots; chemistry; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results
- From: Acta Pharmaceutica Sinica 2006;41(8):747-751
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo establish the RP-HPLC fingerprint analysis for the quality control of Radix Angelicae dahuricae.
METHODSHPLC fingerprint analysis method of Radix Angelicae dahuricae was developed. Kromasil C18 column (250 mm x 4.6 mm ID, 5 microm) was used, with mixture of acetonitrile and water as mobile phase in a gradient mode. The flow rate was 1.0 mL x min(-1). The wavelength of measurement was 254 nm. Twenty-one batches of Radix Angelicae dahuricae were determined.
RESULTSThe 21 samples were classified as 4 clusters by cluster analysis and the 11 superior in producing area samples were confirmed to establish the mutual model. The samples' quality was assessed by Similarity Evaluation System for Chromatographic Fingerprint of TCM 2004.
CONCLUSIONThe method can be used to identify and evaluate the quality of Radix Angelicae dahuricae conveniently.
