Excretion of (-)-clausenamide in rats.
- Author:
Wen QIAN
1
;
Lei-na WANG
;
Min SONG
;
Xiu-wen ZHENG
;
Tai-jun HANG
;
Zheng-xing ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Oral; Animals; Bile; metabolism; Chromatography, High Pressure Liquid; Clausena; chemistry; Feces; chemistry; Female; Lactams; chemistry; pharmacokinetics; urine; Lignans; chemistry; pharmacokinetics; urine; Male; Mass Spectrometry; Neuroprotective Agents; administration & dosage; pharmacokinetics; urine; Plant Leaves; chemistry; Plants, Medicinal; chemistry; Rats; Rats, Sprague-Dawley; Stereoisomerism
- From: Acta Pharmaceutica Sinica 2006;41(8):789-792
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the excretion of (-)-clausenamide in rats.
METHODSThe urine, feces and bile were collected at predetermined time points after (-)-clausenamide was orally administrated to 6 rats (30 mg x kg(-1)). The concentrations of (-)-clausenamide and its metabolite 6-OH-(-)-clausnamide were determined by HPLC-MS/MS method using glipzide as the internal reference, and the accumulative excretion amount of (-)-clausenamide and 6-OH-(-)-clausenamide was calculated in the urine, feces and bile, separately.
RESULTS(-)-Clausenamide was recovered mostly (44%) from feces in 112 hours, 7.1% was found from urine in 120 hours and 0.013% was detected from bile in 24 hours. The accumulative excretions of 6-OH-(-)-clausenamide were 0.92% , 0.46% and 0.0003% of the administered dose from feces, urine and bile, respectively.
CONCLUSIONThe major amount of (-)-clausenamide was recovered from feces after (-)-clausenamide was orally administrated to rats (30 mg kg(-1)).
