Liquid chromatography-tandem electrospray ionization ion trap mass spectrometric assay for the metabolites of jatrorrhizine in rat urine.
- Author:
Feng-mei HAN
1
;
Ming-ming ZHU
;
Huai-xia CHEN
;
Yong CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Berberine; analogs & derivatives; isolation & purification; metabolism; urine; Chromatography, High Pressure Liquid; methods; Coptis; chemistry; Molecular Structure; Plants, Medicinal; chemistry; Rats; Rats, Wistar; Spectrometry, Mass, Electrospray Ionization; methods
- From: Acta Pharmaceutica Sinica 2006;41(9):846-851
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo identify the main metabolites of jatrorrhizine in rat urine.
METHODSThe rat urine samples were collected 0 - 72 h after ig 12 mg x kg(-1) jatrorrhizine, then the samples were purified through C18 solid-phase extraction cartridge. The purified samples were analyzed by combining liquid chromatography and tandem electrospray ionization ion trap mass spectrometry (LC-ESI/ITMS(n)). Identification and structural elucidation of the metabolites were performed by comparing the changes in molecular masses, retention-times and full scan MS(n) spectra with those of the parent drug.
RESULTSAt least seven phase I metabolites (such as de-methyl, de-hydrogen and hydroxyl metabolites) and eleven phase II metabolites (such as glucuronide conjugates and methyl-conjugates) were identified in rat urine.
CONCLUSIONThe developed LC-ESI/ITMS(n) method is not only simple and rapid but also sensitive and specific for the identification of metabolites of jatrorrhizine in rat urine.
