Rapid identification of compounds in cortex moutan by liquid chromatography-tandem mass spectrometry.
- Author:
Shun-jun XU
1
;
Liu YANG
;
Mian ZHANG
;
Zheng-tao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Acetophenones; analysis; chemistry; isolation & purification; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; chemistry; isolation & purification; Glucosides; analysis; chemistry; isolation & purification; Molecular Structure; Monoterpenes; analysis; chemistry; isolation & purification; Paeonia; chemistry; Plant Extracts; analysis; chemistry; isolation & purification; Plants, Medicinal; chemistry; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization; methods; Tandem Mass Spectrometry; methods
- From: Acta Pharmaceutica Sinica 2006;41(9):852-856
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo analyze the chemical constituents of cortex moutan by liquid chromatography coupled with electrospray ionization mass spectrometry.
METHODSAn on-line optimized HPLC-DAD/MS2 technique was employed.
RESULTSIn the negative ion detection mode, 38 components such as monoterpene glucosides, galloylglucoses and acetophenones were isolated. Among them, over thirty compounds were identified, including paeonol, paeonilflorin, oxypaeoniflorin, benzoylpaeoniflorin, benzoyloxypaeoniflorin, galloylpaeoniflorin, galloyloxypaeoniflorin, mundanpioside A, C, D, E, H, etc. by the high performance liquid chromatography with diode-array detection in parallel with electrospray ionization and quadrupole-time of flight tandem mass spectrometry (HPLC-DAD/ESI-MS2).
CONCLUSIONThis method can be used to rapidly determine the constituents of cortex moutan.
