Separation and identification of Taxol in the crude extracts of Taxus cuspidata and its callus culture with HPLC-ESI-MS/MS.
- Author:
Jie ZHANG
1
;
Ji-cheng DUAN
;
Zhen LIANG
;
Wei-bing ZHANG
;
Li-hua ZHANG
;
Yu-shu HUO
;
Yu-kui ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, Liquid; methods; Paclitaxel; chemistry; isolation & purification; Plant Extracts; chemistry; Plants, Medicinal; chemistry; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization; methods; Tandem Mass Spectrometry; Taxus; chemistry
- From: Acta Pharmaceutica Sinica 2006;41(9):863-866
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the MS/MS fragmentation mechanism of Taxol, and based on it to establish HPLC-ESI-MS/MS technique to separate and identify Taxol in the crude extracts of Taxus cuspidata and its callus culture, consequently to provide a fast and credible method for the analysis of Taxol in natural products.
METHODSOptimized the HPLC-ESI-MS/MS parameters for the sample analysis, and then discussed the ionization and cleavage mechanism of Taxol in ESI-MS and ESI-MS/MS, finally identified the Taxol in the samples with retention time, molecular weight and MS/MS spectra.
RESULTSElucidated the MS/MS fragmentation mechanism of Taxol, and developed HPLC-ESI-MS/MS method to analyze Taxol in the two samples.
CONCLUSIONThe HPLC-ESI-MS/MS method is rapid, highly sensitive and specific, so it is suitable for the separation and identification of Taxol in natural products.
