Determination in vitro of rat plasma protein binding rate of sinomenine by using microdialysis method.
- Author:
Ying-feng ZHANG
1
;
Li-ling ZHOU
;
Rui LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood Proteins; metabolism; Chromatography, High Pressure Liquid; Male; Microdialysis; methods; Morphinans; isolation & purification; metabolism; Plants, Medicinal; chemistry; Protein Binding; Rats; Rats, Sprague-Dawley; Regression Analysis; Sinomenium; chemistry
- From: Acta Pharmaceutica Sinica 2006;41(9):909-912
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo determine in vitro the rat plasma protein binding rate by using microdialysis method.
METHODSThe binding rate was determined by using microdialysis probe as sampling tools and zero-net flux method as calibrating method. The regression equation was made by the difference of concentrations between the dialysis sample and the perfusate. The x-intercept of regression equation was the free drug concentration (Cf). The plasma protein binding rate was calculated by using the following equation: f = ( C0 - Cf)/C0.
RESULTThe binding rate was kept relatively stable in the studied concentration range.
CONCLUSIONIt is feasible that the plasma protein binding rate can be determined by using microdialysis method.
