Identification of hydroxylate metabolites of daidzein and its sulfate conjugates in rat urine by LC-ESI/MS(n).
- Author:
Feng-Mei HAN
1
;
Bao-Ying GE
;
Huai-Xia CHEN
;
Yong CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chromatography, Liquid; methods; Hydroxylation; Isoflavones; chemistry; metabolism; urine; Male; Molecular Structure; Phytoestrogens; chemistry; metabolism; urine; Rats; Rats, Sprague-Dawley; Reproducibility of Results; Seeds; chemistry; Soybeans; chemistry; Spectrometry, Mass, Electrospray Ionization; methods; Sulfates; metabolism; Tandem Mass Spectrometry; methods
- From: Acta Pharmaceutica Sinica 2006;41(10):1004-1009
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo identify the hydroxylate metabolites and its sulfate conjugates of daidzein in rat urine.
METHODSUrine samples from 0 - 24 h were collected after single ig dose of 500 mg x kg(-1) daidzein to each of six rats. The urine samples were purified by SPE column (SPE C18) and analyzed with liquid chromatographic-tandem electrospray ionization ion trap mass spectrometry (LC-ESI/MS(n)) for potential metabolites.
RESULTSSeveral new hydroxylate metabolites and its sulfate conjugates were found and identified in rat urine.
CONCLUSIONLC-ESI/MS(n) is proved to be a simple, rapid, sensitive and specific technique for identification of the hydroxylate metabolites and its sulfate conjugates of daidzein in rat urine.