Synthesis of gastrodin by microbial transformation.
- Author:
Hong-li ZHU
1
;
Ji-rong SONG
;
Jian-xin HUANG
;
Jia ZHANG
;
Zhen-Yu MA
;
Ming-yan YANG
Author Information
- Publication Type:Journal Article
- MeSH: Benzaldehydes; chemistry; metabolism; Benzyl Alcohols; chemistry; Biotransformation; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Glucosides; biosynthesis; chemistry; Models, Biological; Molecular Structure; Rhizopus; metabolism
- From: Acta Pharmaceutica Sinica 2006;41(11):1074-1077
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo synthesize gastrodin via biotransformation of microorganism using p-hydroxybenzaldehyde as substrate.
METHODSUsing resting cell techniques with TLC and HPLC analysis, a strain of Rhizopus chinensis Staito AS3. 1165 which has the capability of biotransforming p-hydroxybenzaldehyde into gastrodin was screened from 50 strains of microorganisms. Preparative scale biotransformation was carried out under the optimal transforming condition. The product was obtained by chromatography, and characterized on the basis of its physical and spectral data.
RESULTSThe product was identified as gastrodin on the basis of its 1H NMR, 13C NMR and EI-MS spectral data.
CONCLUSIONThe fact that gastrodin could be synthesized by microbial transformation implies a new approach to gastrodin production. As a result, this research will be of a great economic and social value.
