Prognostic value of methylation status of RASSF1A gene as an independent factor of non-small cell lung cancer.
- Author:
Hui ZHANG
1
;
Shucai ZHANG
;
Zongde ZHANG
;
Hongyan JIA
;
Shuxiang GU
;
Dan ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Carcinoma, Non-Small-Cell Lung; genetics; pathology; DNA Methylation; genetics; Female; Humans; Lung Neoplasms; genetics; pathology; Male; Middle Aged; Prognosis; Promoter Regions, Genetic; genetics; Tumor Suppressor Proteins; genetics
- From: Chinese Journal of Lung Cancer 2010;13(4):311-316
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND AND OBJECTIVEPromoter hypermethylation of the RASSF1A gene is among the most abundant epigenetic deregulations in human cancer. The aim of this study is to investigate the relationship between the methylation status of RASSF1A promoter and the prognoses of non-small cell lung cancer (NSCLC).
METHODSThe methylation status of RASSF1A promoter in 150 NSCLC and 25 non-malignant tissues was determined using a methylation-specific polymerase chain reaction (MSP).
RESULTSRASSF1A promoter hypermethylation was detected in 38.7% (58/150) of NSCLC tissues, but in none of the non-malignant tissues. The patients with hypermethylation of RASSF1A had a poor survival rate, and the relationship between the survival rate and hypermethylation of RASSF1A was statistically significant (P = 0.004). Then by using stepwise Cox proportional hazard regression testing, methylation status of RASSF1A was an independent factor affecting the NSCLC patients' survival (RR = 1.584, 95% CI: 1.040-2.411, P = 0.032).
CONCLUSIONThe hypermethylation of the RASSF1A promoter may be an independent prognostic factor of NSCLC after operation.
