Imbalance of T helper 1 cells/T helper 2 cells accelerated T-cell-mediated endothelium injury in patients with acute coronary syndromes
10.3321/j.issn:0253-3758.2008.12.005
- VernacularTitle:急性冠状动脉综合征患者外周血中辅助性T细胞亚群失衡促进血管内皮细胞的损伤作用
- Author:
Shu-Fang HAN
1
;
Xiao-Yan LI
;
Ke-Wei LIU
;
Lin WANG
;
Rui CUI
;
Wei QIAN
;
Zhan-Peng ZHUO
;
An-Ji ZHANG
Author Information
1. 济南军区总医院
- Keywords:
Coronary disease;
T-lymphocytes;
Endothelial cells
- From:
Chinese Journal of Cardiology
2008;36(12):1070-1073
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the T helper 1 and T helper 2(Th1/Th2)balance and possible association to vascular endothelial cells iniury in patients with acute coronary syndromes(ACS).Methods Forty patients with ACS and l 8 patients with stable angina pectoris(SAP)were included in this study.The concentrations of T helper 1/T helper 2 sabsets related cytokines in plasma were evaluated bv ELISA Kits.Cytotoxic activity of peripheral blood mononuclear cells (PBMCs)or PBMCs depleted CD+ T cells against human umbilical vein endothelial cells(HUVECs)were evaluated by Cr51 cytotoxicity assay.Results Concentrations of T helper 1 related cytokines IFN-γ and IL-2 were significantly higher[IFN-γ:(131.2±42.2)ng/L vs.(47.6±20.2)ng/L;IL-2:(83.7 4±21.3)ng/L vs.(46.2±16.7)ng/I.,all P<0.05]while T helper 2 related cytokine IL-10 concentration was significantly lower[(1 6.7 ±4.3)ng/L vs.(27.5±5.5)ng/L.P<0.05 ] in patients with ACS compared to those in SAP patients.Cytotoxic activity of PBMCs against HUVECs in patients with ACS was also significantly higher than that in patients with SAP (28.84%±4.20% vs.20.28%±2.71%,P<0.05).Conclusions In patients with ACS.Th1 related cytokines were significantly upregulated while Th2 related cytokines were significantly downregulated.This imbalante of Th1/Th2 accelerated PBMCs mediated endothelium iniury in patients with ACS.
